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KMID : 0545120150250030375
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 3 p.375 ~ p.380
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Batch Conversion of Methane to Methanol Using Methylosinus trichosporium OB3b as Biocatalyst
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Hwang In-Yeub
Hur Dong-Hoon
Lee Jae-Hoon
Park Chang-Ho
Chang In-Seop
Lee Jin-Won
Lee Eun-Yeol
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Abstract
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Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30oC. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.
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KEYWORD
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methane, methanol, methanol dehydrogenase, Methylosinus trichosporium OB3b, reducing power
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